منابع مشابه
The structure of DNA in a nucleosome.
We describe the application of the hydroxyl radical footprinting technique to examine the histone-DNA interactions of a nucleosome that includes part of the 5S ribosomal RNA gene of Xenopus borealis. We establish that two distinct regions of DNA with different helical periodicities exist within the nucleosome and demonstrate a change in the helical periodicity of this DNA upon nucleosome format...
متن کاملDNA nanomechanics: how proteins deform the double helix.
It is a standard exercise in mechanical engineering to infer the external forces and torques on a body from a given static shape and known elastic properties. Here we apply this kind of analysis to distorted double-helical DNA in complexes with proteins: We extract the local mean forces and torques acting on each base pair of bound DNA from high-resolution complex structures. Our analysis relie...
متن کاملRSC Unravels the Nucleosome with the DNA
with the DNA. The stability of the nucleosome is reYahli Lorch, Mincheng Zhang, and Roger D. Kornberg* duced, but the gross structure of the nucleosome is Department of Structural Biology unaffected. This “activated” state of the nucleosome is Stanford School of Medicine dependent on the presence of the chromatin-remodelStanford, California 94305 ing complex but not on continuing ATP hydrolysis...
متن کاملDNA Methylation Regulated Nucleosome Dynamics
A strong correlation between nucleosome positioning and DNA methylation patterns has been reported in literature. However, the mechanistic model accounting for the correlation remains elusive. In this study, we evaluated the effects of specific DNA methylation patterns on modulating nucleosome conformation and stability using FRET and SAXS. CpG dinucleotide repeats at 10 bp intervals were found...
متن کاملNucleosome positioning signals in genomic DNA.
Although histones can form nucleosomes on virtually any genomic sequence, DNA sequences show considerable variability in their binding affinity. We have used DNA sequences of Saccharomyces cerevisiae whose nucleosome binding affinities have been experimentally determined (Yuan et al. 2005) to train a support vector machine to identify the nucleosome formation potential of any given sequence of ...
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ژورنال
عنوان ژورنال: Structure
سال: 2009
ISSN: 0969-2126
DOI: 10.1016/j.str.2009.01.013